Anticorps Monoclonal anti-CD146

CD146 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

canin, Humain, Lapin, souris

Applications

FC

Conjugaison

Atlantic Blue™ Fluorescent Dye

CloneNo.

P1H12

N° de cat : AB-65181

Synonymes

CD146, MCAM, MUC18



Applications testées

Résultats positifs en cytométriecellules A375,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

AB-65181 cible CD146 dans les applications de FC et montre une réactivité avec des échantillons canin, Humain, Lapin, souris

Réactivité canin, Humain, Lapin, souris
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène cellules ombilicales humaines cultivées
Nom complet melanoma cell adhesion molecule
Masse moléculaire calculée 646 aa, 72 kDa
Numéro d’acquisition GenBankBC056418
Symbole du gène MCAM
Identification du gène (NCBI) 4162
Conjugaison Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocole

Product Specific Protocols
FC protocol for Atlantic Blue™ CD146 antibody AB-65181Download protocol
Standard Protocols
Click here to view our Standard Protocols