Anticorps Recombinant de lapin anti-CD146/MCAM

CD146/MCAM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250530B11

N° de cat : 86492-1-PBS

Synonymes

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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Galerie de données de validation

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  WB analysis using 86492-1-RR (same clone as 86492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86492-1-RR (MCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86492-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 86492-1-RR (same clone as 86492-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using MCAM antibody (86492-1-RR, Clone: 250530B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86492-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86492-1-PBS cible CD146/MCAM dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD146/MCAM Protéine recombinante Eg2938
• Nom complet: melanoma cell adhesion molecule
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_006500.2
• Symbole du gène: CD146
• Identification du gène (NCBI): 4162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

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