Anticorps Monoclonal anti-CD146/MCAM

CD146/MCAM Monoclonal Antibody for

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4D8A9

N° de cat : CL594-66153

Synonymes

CD146, CD146/MCAM, MCAM, MUC18



Informations sur le produit

CL594-66153 cible CD146/MCAM dans les applications de et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène CD146/MCAM Protéine recombinante Ag11855
Nom complet melanoma cell adhesion molecule
Masse moléculaire calculée 646 aa, 72 kDa
Poids moléculaire observé 120 kDa
Numéro d’acquisition GenBankBC056418
Symbole du gène CD146
Identification du gène (NCBI) 4162
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.

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