Anticorps Polyclonal de lapin anti-CD147

• Phare
• Validé par KD/KO

CD147 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30880-1-PBS

Synonymes

5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN

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Galerie de données de validation

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  WB analysis using 30880-1-AP (same clone as 30880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30880-1-AP (CD147 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 30880-1-AP (same clone as 30880-1-PBS)

  WB result of CD147 antibody (30880-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and CD147 knockout Jurkat cells. This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 30880-1-AP (same clone as 30880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30880-1-AP (CD147 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 30880-1-AP (same clone as 30880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30880-1-AP (CD147 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30880-1-AP (same clone as 30880-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (30880-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30880-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30880-1-PBS cible CD147 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD147 Protéine recombinante Eg0436
• Nom complet: basigin (Ok blood group)
• Masse moléculaire calculée: 385 aa, 42 kDa
• Poids moléculaire observé: 35-55 kDa
• Numéro d’acquisition GenBank: BC009040
• Symbole du gène: CD147
• Identification du gène (NCBI): 682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

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