Anticorps Monoclonal anti-CD147

• Phare
• Validé par KD/KO

CD147 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1G12B5

Avis (1)

N° de cat : 66443-1-Ig

Synonymes

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Galerie de données de validation

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  WB analysis using 66443-1-Ig

  Untreated and PNGase F-treated lysates of TF-1 cells, MOLT-4 cells, Jurkat cells were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66443-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66443-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66443-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66443-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66443-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66443-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66443-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66443-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66443-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 66443-1-Ig

  1X10^6 Jurkat cells were stained with 0.20 ug Anti-Human CD147 (66443-1-Ig, Clone:1G12B5) (red) or 0.20 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone:T1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules TF-1, cellules Jurkat, tissu splénique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'amygdalite humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

66443-1-Ig cible CD147 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD147 Protéine recombinante Ag2619
• Nom complet: basigin (Ok blood group)
• Masse moléculaire calculée: 385 aa, 42 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC009040
• Symbole du gène: BSG
• Identification du gène (NCBI): 682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).

Publications

Species	Application	Title
human	WB	Nat Commun; Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9.; Authors - Mathilde Mathieu
human	WB,IP	Cancer Sci; HSP90 promotes radioresistance of cervical cancer cells via reducing FBXO6-mediated CD147 polyubiquitination.; Authors - Qi Song ; KD Validated
human	WB	Biochim Biophys Acta Mol Cell Res; Crosstalk between pleural mesothelial cell and lung fibroblast contributes to pulmonary fibrosis.; Authors - Fei Liu
human	WB	Anal Bioanal Chem; Novel gene-encoded intermolecular FRET sensor for tracking glycosylation of CD147 in living cells.; Authors - Hui Lu
human	WB	Front Microbiol; Cyclophilin A causes severe fever with thrombocytopenia syndrome virus-induced cytokine storm by regulating mitogen-activated protein kinase pathway; Authors - Huaying Huang
		Colloids Surf B Biointerfaces; Dextran-doxorubicin prodrug nanoparticles conjugated with CD147 monoclonal antibody for targeted drug delivery in hepatoma therapy; Authors - Hongrui Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (03-31-2022)

Seems not good for ARPE-19 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

CD147 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:66443-1-Ig)