Anticorps Recombinant de lapin anti-CD147

CD147 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241852E7

N° de cat : 84488-6-RR

Synonymes

Membrane glycoprotein gp42, HT7 antigen, Bsg, Basigin, Basic immunoglobulin superfamily

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Galerie de données de validation

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  WB analysis using 84488-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84488-6-RR (CD147 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84488-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84488-6-RR (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 84488-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84488-6-RR (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 84488-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84488-6-RR (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84488-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84488-6-RR (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84488-6-RR cible CD147 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: basigin
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42-58 kDa
• Numéro d’acquisition GenBank: NM_009768.2
• Symbole du gène: CD147
• Identification du gène (NCBI): 12215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).

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