Anticorps Monoclonal anti-CD147

CD147 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G12B5

N° de cat : CL488-66443

Synonymes

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL488-66443

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66443 (CD147 antibody) at dilution of 1:50.

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  IF Staining of human tonsillitis using CL488-66443

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66443 (CD147 antibody) at dilution of 1:50.

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  FC experiment of Jurkat using CL488-66443

  1X10^6 Jurkat cells were stained with 0.20ug CD147 antibody (CL488-66443, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en IF: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66443 cible CD147 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD147 Protéine recombinante Ag2619
• Nom complet: basigin (Ok blood group)
• Masse moléculaire calculée: 385 aa, 42 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC009040
• Symbole du gène: BSG
• Identification du gène (NCBI): 682
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).