Anticorps Polyclonal de lapin anti-CD151

CD151 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10418-1-AP

Synonymes

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24

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Galerie de données de validation

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  WB analysis using 10418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10418-1-AP (CD151 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 10418-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CD151 (10418-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, tissu splénique de souris
• Résultats positifs en cytométrie: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10418-1-AP cible CD151 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD151 Protéine recombinante Ag0341
• Nom complet: CD151 molecule (Raph blood group)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001374
• Symbole du gène: CD151
• Identification du gène (NCBI): 977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Publications

Species	Application	Title
rat	WB	FASEB J; Endothelial microparticles induced by cyclic stretch activate Src and modulate cell apoptosis.; Authors - Fei Zhuang
		Int J Mol Med; Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells.; Authors - Deissler Heidrun H

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