Anticorps Polyclonal de lapin anti-CD151

CD151 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27816-1-AP

Synonymes

CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1, MER2

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Galerie de données de validation

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  WB analysis using 27816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27816-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: unboiled A431 cells, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

27816-1-AP cible CD151 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD151 Protéine recombinante Ag25968
• Nom complet: CD151 molecule (Raph blood group)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC001374
• Symbole du gène: CD151
• Identification du gène (NCBI): 977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Publications

Species	Application	Title
human	WB,IHC	Sci Rep; Combined knockdown of CD151 and MMP9 may inhibit the malignant biological behaviours of triple-negative breast cancer through the GSK-3β/β-catenin-related pathway; Authors - Fan Li

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