Anticorps Monoclonal anti-CD151

• Phare
• Validé par KD/KO

CD151 Monoclonal Antibody for WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A8G8

N° de cat : 66567-1-PBS

Synonymes

2A8G8, CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1

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Galerie de données de validation

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  WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

  IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:2000) with human placenta tissue lysate 960 ug. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD151 antibody (66567-1-Ig, Clone: 2A8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50304-1

  Cytometric bead array standard curve of MP50304-1, CD151 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66567-1-PBS. Detection antibody: 66567-2-PBS. Standard:Ag25985. Range: 0.781-50 ng/mL.

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Informations sur le produit

66567-1-PBS cible CD151 dans les applications de WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD151 Protéine recombinante Ag25985
• Nom complet: CD151 molecule (Raph blood group)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC001374
• Symbole du gène: CD151
• Identification du gène (NCBI): 977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

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