Anticorps Recombinant de lapin anti-CD155/PVR

CD155/PVR Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240731B1

N° de cat : 83724-6-RR

Synonymes

Pvr, Tage4, poliovirus receptor, CD155, 240731B1

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Galerie de données de validation

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  WB analysis using 83724-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83724-6-RR (CD155/PVR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 83724-6-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD155/PVR antibody (83724-6-RR, Clone: 240731B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of RAW 264.7 using 83724-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD155/PVR antibody (83724-6-RR, Clone: 240731B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83724-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83724-6-RR against Mouse CD155/PVR were performed. The affinity constant is 0.222 nM.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu d'intestin grêle de souris, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

83724-6-RR cible CD155/PVR dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: poliovirus receptor
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: NM_027514.2
• Symbole du gène: CD155/PVR
• Identification du gène (NCBI): 52118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD155, also known as the poliovirus receptor (PVR) or Necl-5, is one of the nectin-like family members, belonging to the Ca2+ independent immunoglobulin superfamily (IgSF) in cell adhesion molecules (PMID: 37660884). CD155 is a type I transmembrane protein composed of three extracellular immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. It is involved in cell-to-cell and cell-to-ECM adhesion (PMID: 32019260; 11437656). CD155 acts as a ligand for immune receptors, including TIGIT, CD96 and CD226, which are expressed on T cells and NK cells. CD155 interacts with these receptors to modulate immune responses, such as T cell activation and NK cell-mediated cytotoxicity (PMID: 37660884). CD155 serves as the entry receptor for poliovirus and thereby mediates susceptibility to poliovirus infection (PMID: 15034010).

Publications

Species	Application	Title
human	WB,IHC	Sci Adv; Ara-C suppresses H3 K27-altered spinal cord diffuse midline glioma growth and enhances immune checkpoint blockade sensitivity; Authors - Bo Pang

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