Anticorps Recombinant de lapin anti-CD155/PVR

• Phare
• Validé par KD/KO

CD155/PVR Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241405F5

N° de cat : 84138-5-RR

Synonymes

PVR, 241405F5, CD155, HVED, Necl 5

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Galerie de données de validation

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  WB analysis of A549 using 84138-5-RR

  WB result of CD155/PVR antibody (84138-5-RR; 1:1500; room temperature for 1.5 hours) with wild-type and CD155/PVR knockout A549 cells.

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  WB analysis using 84138-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84138-5-RR (CD155/PVR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84138-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84138-5-RR (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84138-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84138-5-RR (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 84138-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD155/PVR antibody (84138-5-RR, Clone: 241405F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84138-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84138-5-RR against Human CD155/PVR were performed. The affinity constant is 2.95 nM.

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules A549, cellules HUVEC, cellules MKN-45
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84138-5-RR cible CD155/PVR dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD155/PVR Protéine recombinante Eg0971
• Nom complet: poliovirus receptor
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC015542
• Symbole du gène: CD155/PVR
• Identification du gène (NCBI): 5817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

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