Anticorps Monoclonal anti-CD155/PVR

CD155/PVR Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

SKII.4

N° de cat : CL488-65251

Synonymes

PVR, CD155, HVED, Necl 5, NECL5

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Galerie de données de validation

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  FC experiment of U-937 using CL488-65251

  U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD155/PVR (CL488-65251, Clone: SKII.4) (red) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124, Clone: MOPC-21) (blue). Cells were not fixed.

• 

  FC experiment of U-937 using CL488-65251

  U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD155/PVR (CL488-65251, Clone: SKII.4) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124, Clone: MOPC-21). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules U-937,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65251 cible CD155/PVR dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SK-N-AS human neuroblastoma cells
• Nom complet: poliovirus receptor
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC015542
• Symbole du gène: CD155/PVR
• Identification du gène (NCBI): 5817
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

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