Anticorps Monoclonal anti-CD16/CD32

CD16/CD32 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, lambda

Réactivité testée

souris

Applications

IF, FC

Conjugaison

Non conjugué

Publications(9)

CloneNo.

93

N° de cat : 65057-1-Ig

Synonymes

CD32, 93, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using 65057-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone: 93) and FITC anti-rat IgG2a Antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: C57BL/6 mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 4 publications below
• FC: See 6 publications below

Informations sur le produit

65057-1-Ig cible CD16/CD32 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B VH81X Tg et lignée de cellules stromales T220
• Nom complet: Fc receptor, IgG, low affinity IIb
• Numéro d’acquisition GenBank: BC038070
• Symbole du gène: CD16/32
• Identification du gène (NCBI): 14130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.

Publications

Species	Application	Title
	FC	Adv Mater; Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction; Authors - Dingyi Wang
mouse	FC	Adv Sci (Weinh); PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer; Authors - Mengdie Cao
mouse	FC	Front Microbiol; Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice; Authors - Beibei Zhang
mouse	IF	Cell Signal; OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization; Authors - Yunyun Li
mouse	IF	Exp Brain Res; Silencing CXCL16 alleviate neuroinflammation and M1 microglial polarization in mouse brain hemorrhage model and BV2 cell model through PI3K/AKT pathway; Authors - Lv Dingyi
mouse	FC	Bioact Mater; Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer; Authors - Jiao Guo

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