Anticorps Monoclonal anti-CD16

CD16 Monoclonal Antibody for FC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

3G8

N° de cat : 65090-1-PBS

Synonymes

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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Galerie de données de validation

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  Sample test of MP50298-3

  Cytometric bead array of Human urine using MP50298-3, CD16a Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Urine of four individual healthy human donors was analyzed using MP50298-3.

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  Sample test of MP50298-4

  Cytometric bead array of Human urine using MP50298-4, CD16a Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Urine of four individual healthy human donors was analyzed using MP50298-4.

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  FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50298-3

  Cytometric bead array standard curve of MP50298-3, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Standard:Eg31662. Range: 1.563-100 ng/mL.

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  Cytometric bead array standard curve of MP50298-4

  Cytometric bead array standard curve of MP50298-4, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Standard:Eg31662. Range: 0.781-100 ng/mL.

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Informations sur le produit

65090-1-PBS cible CD16 dans les applications de FC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

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