Anticorps Monoclonal anti-CD16

CD16 Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1E12E11

N° de cat : 66779-1-PBS

Synonymes

1E12E11, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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Galerie de données de validation

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  IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD16 antibody (66779-1-Ig, Clone: 1E12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66779-1-PBS cible CD16 dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD16 Protéine recombinante Ag9787
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

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