Anticorps Recombinant de lapin anti-CD16

CD16 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242426C2

N° de cat : 98387-1-PBS

Synonymes

CD16A, CD16a antigen, CD16-II, Fc gamma RIII, Fc gamma RIII alpha

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocyte using 98387-1-RR (same clone as 98387-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2) .or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98387-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 98387-1-RR (same clone as 98387-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2)(red).or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG.Cells were not fixed. This data was developed using the same antibody clone with 98387-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 98387-1-RR (same clone as 98387-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2)(red).or Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8 Lot:23015626)(green),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG(red). Cells were not fixed. This data was developed using the same antibody clone with 98387-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98387-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98387-1-RR against Human CD16 were performed. The affinity constant is 12.9 nM.

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Informations sur le produit

98387-1-PBS cible CD16 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD16 Protéine recombinante Eg31662
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

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