Anticorps Recombinant de lapin anti-CD16

CD16 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242426C2

N° de cat : 98387-1-RR

Synonymes

CD16A, CD16a antigen, CD16-II, Fc gamma RIII, Fc gamma RIII alpha

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocyte using 98387-1-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2) .or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using 98387-1-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2)(red).or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG.Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using 98387-1-RR

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD16 Rabbit RecAb (98387-1-RR, Clone:242426C2)(red).or Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8 Lot:23015626)(green),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG(red). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98387-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98387-1-RR against Human CD16 were performed. The affinity constant is 12.9 nM.

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Applications testées

• Résultats positifs en cytométrie: leucocyte de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98387-1-RR cible CD16 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD16 Protéine recombinante Eg31662
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

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