Anticorps Recombinant de lapin anti-CD16

CD16 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Biotin

CloneNo.

3G8

N° de cat : Biotin-65612

Synonymes

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using Biotin-65612

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (Biotin-65612, Clone: 3G8) and CoraLite®488-conjugated streptavidin, or unstained cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using Biotin-65612

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (Biotin-65612, Clone: 3G8) and CoraLite®488-conjugated streptavidin(red), or unstained cells(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-65612 cible CD16 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

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