Anticorps Recombinant de lapin anti-CD16

CD16 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

3G8

N° de cat : PE-65612

Synonymes

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

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Galerie de données de validation

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  FC experiment of human PBMCs using PE-65612

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3, and 5 ul PE Anti-Human CD16 (3G8) Mouse IgG2a RecAb (PE-65612, Clone: 3G8) or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-65612

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD16 (3G8) Mouse IgG2a RecAb (PE-65612, Clone: 3G8) (red) or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65612 cible CD16 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC017865
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

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