Anticorps Monoclonal anti-CD16/CD32

CD16/CD32 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

93

N° de cat : 65057-1-PBS

Synonymes

CD32, 93, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using 65057-1-Ig (same clone as 65057-1-PBS)

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone: 93) and FITC anti-rat IgG2a Antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 65057-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65057-1-PBS cible CD16/CD32 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B VH81X Tg et lignée de cellules stromales T220
• Nom complet: Fc receptor, IgG, low affinity IIb
• Numéro d’acquisition GenBank: BC038070
• Symbole du gène: CD16/32
• Identification du gène (NCBI): 14130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS Only
• Conditions de stockage: Store at -80°C.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.

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