Anticorps Monoclonal anti-KLRB1/CD161

KLRB1/CD161 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Fluorescent Dye

Publications(1)

CloneNo.

HP-3G10

N° de cat : FITC-65115

Synonymes

CD161, CLEC5B, C-type lectin domain family 5 member B, hNKR P1A, HNKR-P1a

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using FITC-65115

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.50 ug APC Anti-Human CD4 APC-65143, Clone: RPA-T4) and 5 ul FITC Anti-Human CD161 (FITC-65115, Clone: HP-3G10). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using FITC-65115

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.50 ug APC Anti-Human CD4 (APC-65143, Clone: RPA-T4) and FITC-mouse IgG1 isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65115 cible KLRB1/CD161 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules NK humaines
• Nom complet: killer cell lectin-like receptor subfamily B, member 1
• Masse moléculaire calculée: 225 aa, 25 kDa
• Numéro d’acquisition GenBank: BC114516
• Symbole du gène: CD161
• Identification du gène (NCBI): 3820
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Publications

Species	Application	Title
		Anal Chem; A High-Efficiency Electrochemical Biosensor for the Detection of Mucosal-Associated Invariant T Cells; Authors - Zhenguo Zhang

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