Anticorps Polyclonal de lapin anti-CD163

CD163 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31024-1-PBS

Synonymes

sCD163, M130

---

Galerie de données de validation

• 

  WB analysis using 31024-1-AP (same clone as 31024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31024-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 31024-1-AP (same clone as 31024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CD163 antibody (31024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CD163 antibody (31024-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

31024-1-PBS cible CD163 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD163 antigen
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: NM_053094.2
• Symbole du gène: Cd163
• Identification du gène (NCBI): 93671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

{{ptg:RelatedPrimaryAntibodies}}