Anticorps Monoclonal anti-CD163

CD163 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G7A5

N° de cat : 68218-1-PBS

Synonymes

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, CD163 molecule

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Galerie de données de validation

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  WB analysis of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68218-1-Ig (CD163 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IP experiment of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  IP result of anti-CD163 (IP:68218-1-Ig, 4ug; Detection:68218-1-Ig 1:4000) with human placenta tissue lysate 1160 ug. This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68218-1-PBS cible CD163 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD163 Protéine recombinante Ag26721
• Nom complet: CD163 molecule
• Masse moléculaire calculée: 1156 aa, 125 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: BC051281
• Symbole du gène: CD163
• Identification du gène (NCBI): 9332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

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