Anticorps Recombinant de lapin anti-CD163

CD163 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240233A3

N° de cat : 83285-4-RR

Synonymes

240233A3, M130, sCD163

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Galerie de données de validation

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  WB analysis using 83285-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 83285-4-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 83285-4-RR

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83285-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 83285-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat small intestine using 83285-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83285-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat, tissu pulmonaire de souris, tissu splénique de rat
• Résultats positifs en IHC: tissu hépatique de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de rat, tissu d'intestin grêle de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83285-4-RR cible CD163 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD163 Protéine recombinante Eg0794
• Nom complet: CD163 antigen
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 160-170 kDa
• Numéro d’acquisition GenBank: NM_053094.2
• Symbole du gène: Cd163
• Identification du gène (NCBI): 93671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

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