Anticorps Monoclonal anti-CD163

CD163 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(6)

CloneNo.

GHI/61

N° de cat : APC-65169

Synonymes

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-65169

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD14 and 5 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61), or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 6 publications below

Informations sur le produit

APC-65169 cible CD163 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Préparation de glycoprotéine à partir de rate de leucémie à cellules velues
• Nom complet: CD163 molecule
• Masse moléculaire calculée: 1156 aa, 125 kDa
• Numéro d’acquisition GenBank: BC051281
• Symbole du gène: CD163
• Identification du gène (NCBI): 9332
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publications

Species	Application	Title
	FC	Mol Cell Probes; Mesenchymal stem cell-derived exosomes promote tissue repair injury in rats with liver trauma by regulating gut microbiota and metabolism; Authors - Bo Yi
	FC	Eur J Pharmacol; Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK; Authors - Xin Wu
human	FC	Front Immunol; Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities; Authors - Chaochao Dai
human	FC	Research (Wash D C); Altered Atlas of Exercise-Responsive MicroRNAs Revealing miR-29a-3p Attacks Armored and Cold Tumors and Boosts Anti-B7-H3 Therapy; Authors - Jie Mei
human	FC	Transl Oncol; Tumor-stroma proportion is associated with increased M2 macrophage abundance and predicts the resistance to immune checkpoint blockade in breast cancer; Authors - Yincheng Liu
human	FC	Life Sci; TPX2 influences the regulation of macrophage polarization via the NF-κB pathway in lung adenocarcinoma; Authors - Lina Wang

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