Anticorps Monoclonal anti-CD163
CD163 Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain
Applications
FC
Conjugaison
FITC Plus Fluorescent Dye
CloneNo.
GHI/61
N° de cat : FITC-65169
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en cytométrie | CMSP humaines, |
Dilution recommandée
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| FC | See 2 publications below |
Informations sur le produit
FITC-65169 cible CD163 dans les applications de FC et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain |
| Hôte / Isotype | Mouse / IgG1, kappa |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Préparation de glycoprotéine à partir de rate de leucémie à cellules velues |
| Nom complet | CD163 molecule |
| Masse moléculaire calculée | 1156 aa, 125 kDa |
| Numéro d’acquisition GenBank | BC051281 |
| Symbole du gène | CD163 |
| Identification du gène (NCBI) | 9332 |
| Conjugaison | FITC Plus Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
| Forme | Liquide |
| Méthode de purification | Purifié par chromatographie d'affinité par protéine A |
| Tampon de stockage | PBS with 0.09% sodium azide and 0.5% BSA |
| Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocole
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD163 antibody FITC-65169 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Tissue Cell Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization | ||
Discov Oncol Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Julie (Verified Customer) (08-26-2025) | Product used at 5uL per 100uL sample containing approximately 4-5 x 10^5 cells. Only shows slight upregulation in IL-10 treated cells, but literature suggests that this is to be expected. Seemed to work better when cells were not PFA fixed.
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