Anticorps Recombinant de lapin anti-CD163

CD163 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

GHI/61

N° de cat : FITC-65522

Synonymes

CD163 molecule, GHI/61, Hemoglobin scavenger receptor, M130, MM130

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using FITC-65522

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone: GHI/61) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65522

  1x10^6 FC receptor block human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone:GHI/61), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44), and 5 ul FITC Plus Anti-Human CD163 (GHI/61) (FITC-65169, Clone: GHI/61), and FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65522 cible CD163 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Préparation de glycoprotéine à partir de rate de leucémie à cellules velues
• Nom complet: CD163 molecule
• Masse moléculaire calculée: 1156 aa, 125 kDa
• Numéro d’acquisition GenBank: BC051281
• Symbole du gène: CD163
• Identification du gène (NCBI): 9332
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

{{ptg:RelatedPrimaryAntibodies}}