Anticorps Monoclonal anti-CD163

CD163 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

Publications

1

CloneNo.

GHI/61

N° de cat : PE-65169

Synonymes

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Galerie de données de validation

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  FC experiment of human peripheral blood monocytes using PE-65169

  0.1 mL human peripheral blood were surface stained with 10 ul FITC-Anti-Human CD14 (PE-65056, Clone: UCHM-1) and 10 ul PE-Anti-Human CD163 (PE-65169, Clone: GHI/61). Sample was then treated with red blood cell lysis buffer.

• 

  FC experiment of human peripheral blood monocytes using PE-65169

  0.1 mL human peripheral blood were surface stained with 10 ul FITC-Anti-Human CD14 (PE-65056, Clone: UCHM-1) and PE-Mouse IgG1 isotype control antibody. Sample was then treated with red blood cell lysis buffer.

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Applications testées

• Résultats positifs en cytométrie: monocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 μl per 10^6 cells in a 100 µl suspension or 10 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

PE-65169 cible CD163 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Préparation de glycoprotéine à partir de rate de leucémie à cellules velues
• Nom complet: CD163 molecule
• Masse moléculaire calculée: 1156 aa, 125 kDa
• Numéro d’acquisition GenBank: BC051281
• Symbole du gène: CD163
• Identification du gène (NCBI): 9332
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publications

Species	Application	Title
human	FC	Cell Death Discov; Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma.; Authors - Tao Yan