Anticorps Recombinant de lapin anti-CD16a

CD16a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Biotin

CloneNo.

242426G11

N° de cat : Biotin-98293

Synonymes

CD16, 242426G11, CD16a antigen, CD16-II, Fc gamma RIII

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-98293

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD16a Rabbit RecAb (Biotin-98293, Clone: 242426G11) and PE-conjugated streptavidin, or unstained (blue). Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56 (B-A19) (CL647-65264, Clone: B-A19). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98293

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD16a Rabbit RecAb (Biotin-98293, Clone: 242426G11) and PE-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-98293 cible CD16a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD16a Protéine recombinante Eg2488
• Nom complet: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Symbole du gène: CD16a
• Identification du gène (NCBI): 2214
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.

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