Anticorps Recombinant de lapin anti-CD16b

CD16b Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

241713E3

N° de cat : PE-98192

Synonymes

CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using PE-98192

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CD16b Rabbit RecAb (PE-98192, Clone: 241713E3) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using PE-98192

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CD16b Rabbit RecAb (PE-98192, Clone: 241713E3) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-98192 cible CD16b dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD16b Protéine recombinante Eg2487
• Nom complet: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Symbole du gène: FCGR3B
• Identification du gène (NCBI): 2215
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).

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