Anticorps Recombinant de lapin anti-CD177

CD177 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241339F4

N° de cat : 98086-1-PBS

Synonymes

NB1, Human neutrophil alloantigen 2a, HNA-2a, HNA2A, HNA 2a

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

  1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98086-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98086-1-RR against Human CD177 were performed. The affinity constant is 1.70 nM.

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Informations sur le produit

98086-1-PBS cible CD177 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD177 molecule
• Masse moléculaire calculée: 437 aa, 46 kDa
• Numéro d’acquisition GenBank: BC029167
• Symbole du gène: CD177
• Identification du gène (NCBI): 57126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).

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