Anticorps Polyclonal de lapin anti-CD18

• Phare
• Validé par KD/KO

CD18 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 10554-1-AP

Synonymes

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

---

Galerie de données de validation

• 

  WB analysis using 10554-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10554-1-AP (CD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 10554-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10554-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 10554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 10554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 10554-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug CD18 antibody (10554-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules U-937,
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• FC: See 2 publications below

Informations sur le produit

10554-1-AP cible CD18 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD18 Protéine recombinante Ag0861
• Nom complet: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC005861
• Symbole du gène: ITGB2
• Identification du gène (NCBI): 3689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Publications

Species	Application	Title
human	FC	J Exp Med; Layilin augments integrin activation to promote antitumor immunity.; Authors - Kelly M Mahuron
pig	IF	Research (Wash D C); Regulating Blood Clot Fibrin Films to Manipulate Biomaterial-Mediated Foreign Body Responses; Authors - Yang Zou
human	WB,IF	ACS Appl Mater Interfaces; Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer.; Authors - Yali Jun
human	WB,IHC,IF	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang ; KD Validated
human	WB	Int J Biol Macromol; Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis; Authors - Feng Jiang
mouse	WB	Cardiovasc Res; Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice.; Authors - Li Liu