Anticorps Recombinant de lapin anti-CD18

CD18 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

TS1/18

N° de cat : 65595-1-MR

Synonymes

ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2

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Galerie de données de validation

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  FC experiment of human PBMCs using 65595-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD45 (HI30) (APC-65109, Clone: HI30). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65595-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65595-1-MR cible CD18 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Intégrine bêta 2 humaine
• Nom complet: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Masse moléculaire calculée: 85 kDa
• Numéro d’acquisition GenBank: BC005861
• Symbole du gène: CD18
• Identification du gène (NCBI): 3689
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

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