Anticorps Monoclonal anti-CD18

CD18 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Fluorescent Dye

CloneNo.

M18/2

N° de cat : FITC-65178

Synonymes

Itgb2, integrin beta 2, LAD, LCAMB, Lfa1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using FITC-65178

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD18 (FITC-65178, Clone: M18/2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65178

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD18 (FITC-65178, Clone: M18/2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65178 cible CD18 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glycoprotéine CTL de souris
• Nom complet: integrin beta 2
• Numéro d’acquisition GenBank: BC145644
• Symbole du gène: CD18
• Identification du gène (NCBI): 16414
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'isothiocyanate de fluorescéine (FITC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

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