Anticorps Recombinant de lapin anti-CD19

CD19 Recombinant Antibody for FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

1D3

N° de cat : 65661-1-PBS

Synonymes

CD19 antigen

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 65661-1-TR (same clone as 65661-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone:1D3) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 65661-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 65661-1-TR (same clone as 65661-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone: 1D3) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65661-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65661-1-PBS cible CD19 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD19 antigen
• Numéro d’acquisition GenBank: BC156767
• Symbole du gène: Cd19
• Identification du gène (NCBI): 12478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

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