Anticorps Monoclonal anti-CD19

CD19 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C10A1

N° de cat : 66298-1-PBS

Synonymes

1C10A1, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

---

Galerie de données de validation

• 

  WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 66298-1-Ig (same clone as 66298-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66298-1-Ig (same clone as 66298-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (66298-1-Ig, Clone: 1C10A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66298-1-PBS cible CD19 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD19 Protéine recombinante Ag19945
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

{{ptg:RelatedPrimaryAntibodies}}