Anticorps Recombinant de lapin anti-CD19

CD19 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242301B9

N° de cat : 84848-5-PBS

Synonymes

242301B9, B-lymphocyte antigen CD19, CD19 antigen

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Galerie de données de validation

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  WB analysis of mouse splenocytes using 84848-5-RR (same clone as 84848-5-PBS)

  mouse splenocytes were subjected to SDS PAGE followed by western blot with 84848-5-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84848-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84848-5-RR (same clone as 84848-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84848-5-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84848-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84848-5-RR (same clone as 84848-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84848-5-RR (CD19 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84848-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84848-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84848-5-RR against Mouse CD19 were performed. The affinity constant is 6.90 nM.

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Informations sur le produit

84848-5-PBS cible CD19 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD19 Protéine recombinante Eg3194
• Nom complet: CD19 antigen
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 80-120 kDa
• Numéro d’acquisition GenBank: NM_009844.2
• Symbole du gène: Cd19
• Identification du gène (NCBI): 12478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

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