Anticorps Recombinant de lapin anti-CD19

CD19 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250499C1

N° de cat : 86116-1-PBS

Synonymes

B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CD19 molecule

---

Galerie de données de validation

• 

  WB analysis using 86116-1-RR (same clone as 86116-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 89985-2-RR (CD19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86116-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 86116-1-RR (same clone as 86116-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86116-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86116-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 86116-1-RR (same clone as 86116-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86116-1-RR (CD19 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86116-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 86116-1-RR (same clone as 86116-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD19 antibody (86116-1-RR, Clone: 250499C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86116-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 86116-1-RR (same clone as 86116-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD19 antibody (86116-1-RR, Clone: 250499C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86116-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

86116-1-PBS cible CD19 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD19 Protéine recombinante Eg3335
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

{{ptg:RelatedPrimaryAntibodies}}