Anticorps Recombinant de lapin anti-CD19

CD19 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Biotin

CloneNo.

4G7

N° de cat : Biotin-65562

Synonymes

4G7, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-65562

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD19 (4G7) Mouse IgG2a RecAb (Biotin-65562, Clone:4G7) and PE-conjugated streptavidin, or unstained. Cells were co-stained with 5 ul CoraLite® Plus 647 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CL647-65570, Clone: UCHT1). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65562

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD19 (4G7) Mouse IgG2a RecAb (Biotin-65562, Clone:4G7) and PE-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-65562 cible CD19 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules humaines de leucémie lymphoïde chronique (LLC)
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

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