Anticorps Monoclonal anti-CD2

CD2 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A10B2

N° de cat : 60005-1-PBS

Synonymes

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

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Galerie de données de validation

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  WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 60005-1-Ig (same clone as 60005-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51114-3

  Cytometric bead array standard curve of MP51114-3, CD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60005-1-PBS. Detection antibody: 60781-4-PBS. Standard:Eg0138. Range: 6.25-100 ng/mL.

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Informations sur le produit

60005-1-PBS cible CD2 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD2 Protéine recombinante Ag0423
• Nom complet: CD2 molecule
• Masse moléculaire calculée: 351 aa, 39 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC033583
• Symbole du gène: CD2
• Identification du gène (NCBI): 914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

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