Anticorps Recombinant de lapin anti-CD2

CD2 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241222B8

N° de cat : 84108-5-PBS

Synonymes

241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

---

Galerie de données de validation

• 

  WB analysis using 84108-5-RR (same clone as 84108-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 84108-5-RR (same clone as 84108-5-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01045-4

  Sandwich ELISA standard curve of MP01045-4, Human CD2 Recombinant Matched Antibody Pair - PBS only. 84108-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1271. 84108-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

• 

  Sample test of MP01045-4

  Serum of six individual healthy human donors was measured. The human CD2 concentration of detected samples was determined to be 167.0 pg/mL with a range of 119.9 - 223.9 pg/mL.

• 

  Sample test of MP01045-4

  Jurkat and Molt-4 cell lysate were measured. The human CD2 concentration of detected samples was determined to be 8,318.1 pg/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate and 679.3 pg/mL (based on a 4.5 mg/mL extract load) in Molt-4 cell lysate.

• 

  IF Staining of Jurkat using 84108-5-RR (same clone as 84108-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat (positive) Raji (negative) cells using CD2 antibody (84108-5-RR, Clone: 241222B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84108-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

---

Informations sur le produit

84108-5-PBS cible CD2 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD2 Protéine recombinante Eg1271
• Nom complet: CD2 molecule
• Masse moléculaire calculée: 351 aa, 39 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC033583
• Symbole du gène: CD2
• Identification du gène (NCBI): 914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

{{ptg:RelatedPrimaryAntibodies}}