Anticorps Recombinant de lapin anti-CD2

CD2 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241548D7

N° de cat : 84360-4-PBS

Synonymes

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

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Galerie de données de validation

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  WB analysis using 84360-4-RR (same clone as 84360-4-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01249-1

  Sandwich ELISA standard curve of MP01249-1, Mouse CD2 Recombinant Matched Antibody Pair - PBS only. 84360-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1403. 84360-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP01249-1

  The mean CD2 concentration was determined to be 3.0 ng/mL in CTLL-2 cell extract based on a 0.9 mg/mL extract load.

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  IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01249-1

  Cytometric bead array standard curve of MP01249-1, MOUSE CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84360-5-PBS. Detection antibody: 84360-4-PBS. Standard: Eg1403. Range: 0.313-40 ng/mL.

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Informations sur le produit

84360-4-PBS cible CD2 dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FusionProtein
• Nom complet: CD2 antigen
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_013486.2
• Symbole du gène: CD2
• Identification du gène (NCBI): 12481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

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