Anticorps Recombinant de lapin anti-CD2

CD2 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241222E2

N° de cat : 98134-1-RR

Synonymes

241222E2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

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Galerie de données de validation

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  FC experiment of human PBMCs using 98134-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit RecAb (98134-1-RR, Clone: 241222E2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using 98134-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit RecAb (98134-1-RR, Clone: 241222E2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98134-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98134-1-RR against Human CD2 were performed. The affinity constant is 0.418 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98134-1-RR cible CD2 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD2 Protéine recombinante Eg1271
• Nom complet: CD2 molecule
• Masse moléculaire calculée: 351 aa, 39 kDa
• Numéro d’acquisition GenBank: BC033583
• Symbole du gène: CD2
• Identification du gène (NCBI): 914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

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