Anticorps Polyclonal de lapin anti-CD20

CD20 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31909-1-PBS

Synonymes

Membrane-spanning 4-domains subfamily A member 1, Lymphocyte antigen 44, B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44

---

Galerie de données de validation

• 

  WB analysis using 31909-1-AP (same clone as 31909-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31909-1-AP (CD20 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

31909-1-PBS cible CD20 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD20 Protéine recombinante Ag36918
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_007641
• Symbole du gène: Ms4a1
• Identification du gène (NCBI): 12482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

{{ptg:RelatedPrimaryAntibodies}}