Anticorps Recombinant de lapin anti-CD20

CD20 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Non conjugué

CloneNo.

2H7

N° de cat : 65575-1-PBS

Synonymes

2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1

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Galerie de données de validation

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  FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

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  FC experiment of Cnyo PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD20 (2H7) Mouse IgG2a RecAb (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Cnyo PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD20 (2H7) Mouse IgG2a RecAb (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone:2H7)(left) or 0.25 ug Anti-Human CD20 (65085-1-Ig, Clone: 2H7)(right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65575-1-PBS cible CD20 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lymphocytes B amygdaliennes humaines
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: CD20
• Identification du gène (NCBI): 931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

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