Anticorps Recombinant de lapin anti-CD20

CD20 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Biotin

CloneNo.

2H7

N° de cat : Biotin-65575

Synonymes

2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-65575

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody (Biotin-65575, Clone: 2H7) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using Biotin-65575

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody (Biotin-65575, Clone: 2H7) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-65575 cible CD20 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lymphocytes B amygdaliennes humaines
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: CD20
• Identification du gène (NCBI): 931
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

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