Anticorps Monoclonal anti-CD20

CD20 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / Mouse IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

2H7

N° de cat : CL405-65085

Synonymes

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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Galerie de données de validation

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  FC experiment of human PBMCs using CL405-65085

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul CoraLite® Plus 405 Anti-Human CD20 (CL405-65085, Clone:2H7), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65085

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD20 (CL405-65085, Clone:2H7), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-65085 cible CD20 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / Mouse IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B amygdaliennes humaines
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: MS4A1
• Identification du gène (NCBI): 931
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).