Anticorps Monoclonal anti-CD200

• Phare
• Validé par KD/KO

CD200 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5F3D6

N° de cat : 66282-1-PBS

Synonymes

CD200 molecule, MOX1, MOX2, MRC, OX 2

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Galerie de données de validation

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  WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

  Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66282-1-Ig (same clone as 66282-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66282-1-PBS cible CD200 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD200 Protéine recombinante Ag5045
• Nom complet: CD200 molecule
• Masse moléculaire calculée: 269 aa, 30 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC022522
• Symbole du gène: CD200
• Identification du gène (NCBI): 4345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

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