Anticorps Recombinant de lapin anti-CD200

CD200 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241329G4

N° de cat : 98091-2-RR

Synonymes

241329G4, CD200 molecule, MOX1, MOX2, MRC

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 98091-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit RecAb (98091-2-RR, Clone: 241329G4) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98091-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit RecAb (98091-2-RR, Clone: 241329G4) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98091-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98091-2-RR against Human CD200 were performed. The affinity constant is 0.405 nM.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98091-2-RR cible CD200 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD200 molecule
• Masse moléculaire calculée: 269 aa, 30 kDa
• Numéro d’acquisition GenBank: BC022522
• Symbole du gène: CD200
• Identification du gène (NCBI): 4345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

{{ptg:RelatedPrimaryAntibodies}}